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KMID : 0895820220320020001
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Journal of Oriental Rehabilitation Medicine
2022 Volume.32 No. 2 p.1 ~ p.17
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A Study on β-glucan, Ginsenoside Content, 2,2-diphenyl-1-picrylhydrazyl Free Radical Scavenging Activity, Anti-inflammatory Activity and Safety of Herbal Medicine Mix - Iksooyoungjingogami with Scutellariae Radix and Houttuynia cordata Thunb
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Kim Myeong-Hun
Moon Yang-Seon
Kang Sang-Mi
Kim Heyong-Seok
Kim Seon-Jong
Na Chang-Su
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Abstract
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Objectives This study was conducted to investigate the beta-glucan & ginsenoside content, antioxidant activity, anti-inflammatory effect and safety of herbal medicine mix.
Methods The marker compounds contents, antioxidant activity and safety of herbal medicine mix were tested. The contents of beta-glucan and ginsenoside Rg3 were measured, the antioxidant activity was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, anti-inflammatory and a safety test was conducted via single dose toxicity assessment.
Results Analyzing the contents of marker compounds showed 362.3 mg/g of beta- glucan, and 0.4184 mg/g of ginsenoside Rg3. In the DPPH free radical scavenging activity, the IC50 of herbal medicine mix, was 0.146%. The scavenging activity of herbal medicine mix was 88.28% activity at 0.5% concentration, and 90.61% activity at 5% concentration. In the lipopolysaccharides (LPS) anti-inflammatory test, the herbal remix showed a significant decrease in tumor necrosis factor-alpha (TNF-α) and interleukin- 6 (IL-6) compared to the LPS-induced group. In the single dose toxicity test of herbal medicine mix, a dose of 2,000 mg/kg body weight (BW) was set at its highest capacity and observed after oral administration to female and male rats. No toxicological findings were recognized. It was observed that the resulting lethal dose can be set to 2,000 mg/kg BW or higher for both females and males.
Conclusions The results of the experiment on herbal medicine mix showed that the marker compounds contents were beta-glucan and ginsenoside Rg3, that antioxidant activity was observed through the DPPH free radical scavenging activity, anti-inflammatory effect was observed through TNF-α and IL-6 measurement, and safety was confirmed through the single dose toxicity assessment.
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KEYWORD
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Herbal medicine, Beta-glucans, Ginsenoside Rg3, 1, 1-diphenyl-2-picrylhydrazyl, Tumor necrosis factor-alpha, Interleukin-6
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